User:Eleni N. Kalivas/Notebook/CHEM-571/2014/04/08: Difference between revisions
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# The solution is stirred for 20 minutes | # The solution is stirred for 20 minutes | ||
# The solution is then aged at 50C under air for 2 hours. | # The solution is then aged at 50C under air for 2 hours. | ||
==Stock Solution Prep== | |||
The solutions created for 78%BSA by mass with L-Cysteine synthesis: | |||
# 250mL of 20mM L-cysteine | |||
## FW 121.16g/mol | |||
## measure out: 0.6058g | |||
## measured: 0.6072g | |||
## actual conc: 20.05mM | |||
# 50mL of 100mM ZnSO<sub>4</sub> | |||
## FW 287.56g/mol | |||
## measure out: 1.4378g | |||
## measured: 1.4399 g | |||
## actual conc: 100.1 mM | |||
# 50mL of 10mM MnCl<sub>2</sub> | |||
## FW 197.9g/mol | |||
## measure out: 0.09895g | |||
## measured: 0.0987g | |||
## actual conc: 9.98 mM | |||
# 10mL 1M NaOH | |||
## FW 40.00g/mol | |||
## 0.4284g | |||
## Final concentration a bit over 1M check pH carefully when adjusting! | |||
# 25mL 100mM Na<sub>2</sub>S | |||
## FW 240.18g/mol | |||
## measure out: 0.600g | |||
## measured: 0.610g | |||
## actual conc: 101.6 mM | |||
# 25mL .1484 mM BSA | |||
Revision as of 12:15, 15 April 2014
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Objective
ProtocolThis protocol was taken from Matt Harting's notebook, all solutions de-gassed before synthesis.
Stock Solution PrepThe solutions created for 78%BSA by mass with L-Cysteine synthesis:
|