User:Eleni N. Kalivas/Notebook/CHEM-571/2014/10/01: Difference between revisions
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*Fluorescence of PVA/PVAC films and glass absorbance standards | *Fluorescence of PVA/PVAC films and glass absorbance standards | ||
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Revision as of 10:55, 3 October 2014
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Objective
ProcedurePreparation of stock solutions0.0120g of R6G in 10mL needed to make the initial stock solution of 2500uM, this was used to prepare a secondary stock of 500uM, used to make the dilutions for 0.1, 0.5, 0.75, 1.0, 1.2 and 1.5. The initial stock of 2500uM was used to make a 2uM. But the 1.5 and 2 uM were not used for the calibration curve because the fluorescence was too high that it did not provide accurate data.
Fluorescence
UV-Vis
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