User:Eleni N. Kalivas/Notebook/CHEM-571/2015/01/28: Difference between revisions

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(Autocreate 2015/01/28 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571)
 
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==Entry title==
==Objective==
* Insert content here...
First trial of synthesizing PVA-Alginate beads with and without NaMT
 
==Protocol==
*Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
## 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
## ~0.1 g of nanocomposites should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 2% calcium chloride
## Add 4.2 grams of calcium chloride to 210 mL of water
## Add citric acid to saturation
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
# Stir for 24hrs to allow solidification
# Rinse with water to remove excess reactants
 




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Objective

First trial of synthesizing PVA-Alginate beads with and without NaMT

Protocol

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C
    1. 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
    2. ~0.1 g of nanocomposites should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 2% calcium chloride
    1. Add 4.2 grams of calcium chloride to 210 mL of water
    2. Add citric acid to saturation
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle
  5. Stir for 24hrs to allow solidification
  6. Rinse with water to remove excess reactants



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