User:Eleni N. Kalivas/Notebook/CHEM-571/2015/04/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span> | ||
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== | ==April 3, 2015== | ||
* | *absorption | ||
*FTIR | |||
*large scale synthesis | |||
===Procedure=== | |||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)], [http://ac.els-cdn.com/S135951130700342X/1-s2.0-S135951130700342X-main.pdf?_tid=9b1dd946-dfb1-11e4-8f3d-00000aab0f26&acdnat=1428691643_41053c22f2159065885cfb5d19ec028f | Idirs et al.] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.> | |||
<br.> | |||
PVA-Alg-NaMT | |||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | |||
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate) | |||
#** Add alginate '''while stirring''' PVA-water solution | |||
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of citric acid, calcium chloride solution | |||
## Add '''12 g (5%) citric acid''' | |||
## Add '''8.4 g (4%) calcium chloride''' | |||
## Heat solution to 40°C while stirring | |||
# Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip | |||
# Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution | |||
# Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture. | |||
#Allow solution to cool to room temp while stirring for another 20 min | |||
# Stir in '''24 g''' (10%) citric acid solution for 30 min | |||
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 10 min | |||
# Rinse with water to remove excess reactants | |||
# Allow beads to dry in teflon plate overnight | |||
# Clean syringe | |||
#* Pass syringe tip over flame, solution will burst out | |||
#* Use syringe to push water through tip to get any remaining solution out | |||
#* Rinse again with water until solution runs clear | |||
<br.> | |||
<br.> | |||
Malachite Green for Absorption | |||
*2000 ppm stock actual concentration (calculated): 1523 ppm | |||
**0.5253 mL 1523 ppm stock in 10 mL to make 80 ppm solution | |||
**152.3 ppm stock from last time to make 200 ppm solution | |||
**52.53 uL 152.3 ppm stock in 10 mL to make 8 ppm solution | |||
**0.1313 mL 152.3 ppm stock in 10 mL to make 2 ppm solution | |||
<br.> | |||
<br.> | |||
Rhodamine 6G for Absorption | |||
*1 mL 1 mM R6G in 5 mL to make 0.02 mM solution | |||
====Measurements==== | |||
*PVA: | |||
*Alg: | |||
*NaMT: | |||
*citric acid: | |||
*CaCl2 | |||
*citric acid: | |||
*sodium sulfate: | |||
====Notes==== | |||
*beads rehydrate so quickly! | |||
*run at least one XRD sample at VERY high resolution with expanded range | |||
*run XRD of plain PVA (20-25), Alg, and NaMT (8, 6 > when inter-colated; we have: 8.5, 20, 28, 35.5) | |||
**look up pure PVA crystalline XRD to see if we have any in our structure | |||
*Imaging of R6G beads | |||
*observation of absorption beads: about half the beads absorb malachite green/R6G (seen by color change) and half remain white. the beads that absorb float while the one that don't sink. | |||
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Revision as of 06:14, 17 April 2015
PVA-Alg Bead Synthesis | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 3, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992), | Idirs et al. and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT
<br.> <br.> Malachite Green for Absorption
<br.> <br.> Rhodamine 6G for Absorption
Measurements
Notes
|