User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/09/21: Difference between revisions
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==Objective== | ==Objective== | ||
To preform electrophoresis using an agarose gel to determine if the PCR performed the previous day successfully mutated the GFP protein. | |||
To carry out protein expression using the starter culture media and the expression culture media prepared the day before. | |||
==Description== | ==Description== | ||
<u>Electrophoresis Procedure</u> | |||
# A 1% agarose gel was prepared using 0.25 g agarose and 25 mL of 1X TAE buffer. | |||
# The flask containing the agarose and 1X TAE buffer was microwaved for 40 seconds. | |||
# The gel casting tray was assembled and the comb was placed in the tray. | |||
# The agarose solution was poured into the mold and allowed to sit until it had cooled and solidified. | |||
# Once the gel had solifified, the comb was gently removed. | |||
# The gel was placed on the center of the electrophoresis chamber. | |||
# The reservoirs surrounding the gel were filled with additional TAE buffer. The gel was submerged in buffer about one centimeter. | |||
# The DNA samples were prepared for the gel by mixing 1 μL of loading dye with 5μL of PCR product. | |||
# 6 μL of each sample was added to each gel wall, beginning with a DNA ladder in the first well on the left. | |||
==Data== | ==Data== |
Revision as of 14:30, 27 September 2011
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ObjectiveTo preform electrophoresis using an agarose gel to determine if the PCR performed the previous day successfully mutated the GFP protein. To carry out protein expression using the starter culture media and the expression culture media prepared the day before. DescriptionElectrophoresis Procedure
Data
NotesThis area is for any observations or conclusions that you would like to note.
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