User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/05: Difference between revisions
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The intensity values for each solution were corrected against the blank of 50 mM Tris (pH 7.55). The corrected intensities were graphed against the wavelengths. The intensity values of some of the high MBP concentrations were so high that they exceeded the maximum intensity that could be read by the fluorimeter. | The intensity values for each solution were corrected against the blank of 50 mM Tris (pH 7.55). The corrected intensities were graphed against the wavelengths. The intensity values of some of the high MBP concentrations were so high that they exceeded the maximum intensity that could be read by the fluorimeter. | ||
The corrected intensities calculated for each MBP solution were then divided by the concentration of the solution. The corrected intensities divided by the concentrations were graphed against the wavelengths for each solution. | |||
[[Image:Solution_A_Corrected_Intensity_Divided_by_Concentration_vs._Wavelength.png]] | |||
[[Image:Solution_B_Corrected_Intensity_Divided_by_Wavelength.png]] | |||
[[Image:Solution_C_Corrected_Intensity_Divided_by_Concentration_vs._Wavelength.png]] | |||
[[Image:Solution_D_Corrected_Intensity_Divided_by_Concentration_vs._Wavelength.png]] | |||
[[Image:Solution_E_Corrected_Intensity_Divided_by_Concentration_vs._Wavelength.png]] | |||
Revision as of 13:29, 19 October 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo determine the fluorescence of MBP as a function of the concentration and to purify the DNA made from previously cultured cells. Description
DataThe intensity values for each solution were corrected against the blank of 50 mM Tris (pH 7.55). The corrected intensities were graphed against the wavelengths. The intensity values of some of the high MBP concentrations were so high that they exceeded the maximum intensity that could be read by the fluorimeter.
NotesThis area is for any observations or conclusions that you would like to note.
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