User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/25: Difference between revisions
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==Description== | ==Description== | ||
# The stacking gel was prepared by miing the following in an Erlenmeyer flask: 17 mL of H<sub>2</sub>O, 4.15 mL of 30% arylamide, 3.15 mL of 1.0M Tris (pH 6.8), 0.25 mL of 10% SDS. | |||
# The following | # Once the pre-made resolving gel solution had set (after 20 minutes), 0.25 mL of 10% ammonium persulfate and 0.025 mL of TEMED were added to the stacking gel solution. | ||
# The stacking gel solution was the poured on top of the resolving gel and allowed to set for 20 minutes. | |||
# The four samples to be loaded into the the protein gel were prepared as follows: | |||
==Data== | ==Data== |
Revision as of 11:05, 8 November 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo run a protein gel to determine if the MBP-intein fusion has been expressed and purified. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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