User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/25: Difference between revisions
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==Objective== | ==Objective== | ||
To run a protein gel to determine if the MBP-intein fusion has been expressed and purified. | To run a protein gel (SDS-PAGE) to determine if the MBP-intein fusion has been expressed and purified. | ||
==Description== | ==Description== | ||
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==Data== | ==Data== | ||
[[Image:Group2.JPG]] | |||
[[Image:AllGels.JPG]] | |||
==Notes== | ==Notes== | ||
* Protein was present, but the sample was impure. Purification was not successful. | |||
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. | Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. |
Revision as of 15:06, 3 December 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo run a protein gel (SDS-PAGE) to determine if the MBP-intein fusion has been expressed and purified. Description
Tube 1 - 10 μL of ladder mixed with 4 μL of orange dye Tube 2 - 16 μL of protein from Peak 1 with 4 μL of orange dye Tube 3 - 16 μL of protein from Peak 2 with 4 μL of orange dye Tube 4 - 16 μL of protein from Peak 3 with 4 μL of orange dye DataNotes
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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