User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/08: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
(2 intermediate revisions by the same user not shown) | |||
Line 10: | Line 10: | ||
==Objective== | ==Objective== | ||
To synthesize gold nanoparticles from BSA and HAuCl<sub>4</sub>. | To synthesize gold nanoparticles from BSA and HAuCl<sub>4</sub>. A mini prep was also performed. | ||
==Description== | ==Description== | ||
# 100 μL of BSA and 100μL of HAuCl<sub>4</sub> were mixed in an epitube. Two reaction mixtures were made, labeled A and B. | # 100 μL of BSA (15.5 uM) and 100μL of HAuCl<sub>4</sub> (2.9 mM) were mixed in an epitube. Two reaction mixtures were made, labeled A and B. | ||
# The reaction mixtures were placed in a heat block set to 70°C. Tube A was taken out of the heat block for 10 minutes every 30 minutes, while Tube B was left in the heat block for the entire reaction time. | # The reaction mixtures were placed in a heat block set to 70°C. Tube A was taken out of the heat block for 10 minutes every 30 minutes, while Tube B was left in the heat block for the entire reaction time. | ||
<u>Mini Prep</u> | |||
# 5 mL of LB were placed in a tube. | |||
# 5 μL of ampicillin were added to each tube. | |||
# 1 colony was added to each tube. | |||
# The tubes were placed on the shaker. | |||
==Data== | ==Data== | ||
* | * At the end of the reaction, Tube A hard a dark purple clump at the bottom of the reaction tube. Tube B had purple fibers floating in the middle of the tube. | ||
* Final concentration of BSA in both reactions was 1.55 uM. The final concentration of HAuCl<sub>4</sub> was 0.29 mM in both reaction mixtures. | |||
==Notes== | ==Notes== |
Revision as of 15:39, 3 December 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo synthesize gold nanoparticles from BSA and HAuCl4. A mini prep was also performed. Description
Mini Prep
Data
NotesThis area is for any observations or conclusions that you would like to note.
|