User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png"border="0" /></html>}} | ||
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* Tube A - A 1.5 mL epitube was used. 800 μL of water was added to the BSA and HAuCl<sub>4</sub>. There was air present on top of the solution. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature. | * Tube A - A 1.5 mL epitube was used. 800 μL of water was added to the BSA and HAuCl<sub>4</sub>. There was air present on top of the solution. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature. | ||
* Tube B - A 600 uL epitube was used with 600 μL of water. There was no space between the top of the solution and the top of the tube. | * Tube B - A 600 uL epitube was used with 600 μL of water. There was no space between the top of the solution and the top of the tube. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature. | ||
* Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution. At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced. | * Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution. At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced. | ||
*Tube D - | *Tube D - A 600 uL epitube was used with 600 μL of water, so that there was no space between the solution and the top of the tube. Once the reaction time was over, the | ||
Revision as of 13:38, 9 November 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png"border="0" /></html> |
ObjectiveTo synthesize gold nanoparticles while controlling the volume of air in contact with the solution, the volume of water used, and the cooling of the reaction mixture. The mini preps were also worked up today. DescriptionNanoparticle Synthesis 7 reaction mixtures were set up, each with varying conditions. Each tube contained 1 μL of BSA, 1 μL of HAuCl4 and had water as the solvent. The reaction mixtures were placed in the heat block for 2 hours at 80°C.
Work Up
Data
NotesThis area is for any observations or conclusions that you would like to note.
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