User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/09: Difference between revisions

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* Tube B -  A 600 uL epitube was used with 600 μL of water.  There was no space between the top of the solution and the top of the tube. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
* Tube B -  A 600 uL epitube was used with 600 μL of water.  There was no space between the top of the solution and the top of the tube. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
* Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution.  At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced.
* Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution.  At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced.
*Tube D - A 600 uL epitube was used with 600 μL of water, so that there was no space between the solution and the top of the tube. Once the reaction time was over, the  
* Tube D - A 600 uL epitube was used with 600 μL of water, so that there was no space between the solution and the top of the tube. Once the reaction time was over, the tube was left in the heat block while it cooled to room temperature
* Tube E - A 1.5 mL tube was used and 800 μL of water was added to the tube. The top of the tube was opened every 30 minutes, exposing the tube to the air. This tube was removed from the heat block when the reaction time was over. 
* Tube F -
 




Revision as of 13:44, 9 November 2011

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Objective

To synthesize gold nanoparticles while controlling the volume of air in contact with the solution, the volume of water used, and the cooling of the reaction mixture. The mini preps were also worked up today.

Description

Nanoparticle Synthesis 7 reaction mixtures were set up, each with varying conditions. Each tube contained 1 μL of BSA, 1 μL of HAuCl4 and had water as the solvent. The reaction mixtures were placed in the heat block for 2 hours at 80°C.

  • Tube A - A 1.5 mL epitube was used. 800 μL of water was added to the BSA and HAuCl4. There was air present on top of the solution. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
  • Tube B - A 600 uL epitube was used with 600 μL of water. There was no space between the top of the solution and the top of the tube. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
  • Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution. At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced.
  • Tube D - A 600 uL epitube was used with 600 μL of water, so that there was no space between the solution and the top of the tube. Once the reaction time was over, the tube was left in the heat block while it cooled to room temperature
  • Tube E - A 1.5 mL tube was used and 800 μL of water was added to the tube. The top of the tube was opened every 30 minutes, exposing the tube to the air. This tube was removed from the heat block when the reaction time was over.
  • Tube F -



Work Up


Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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