User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/09

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Objective

To synthesize gold nanoparticles while controlling the volume of air in contact with the solution, the volume of water used, and the cooling of the reaction mixture.

Description

Nanoparticle Synthesis

7 reaction mixtures were set up, each with varying conditions. Each tube contained 100 μL of BSA (15.5 uM) , 100 μL of HAuCl4 (2.5 mM), and had water as the solvent. The reaction mixtures were placed in the heat block for 2 hours at 80°C.

  • Tube A - A 1.5 mL epitube was used. 800 μL of water was added to the BSA and HAuCl4. There was air present on top of the solution. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
  • Tube B - A 600 uL epitube was used with 600 μL of water. There was no space between the top of the solution and the top of the tube. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
  • Tube C - A 1.5 mL tube was used. 800 μL of water was added and there was space between the top of the tube and the top of the solution. At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced.
  • Tube D - A 600 uL epitube was used with 600 μL of water, so that there was no space between the solution and the top of the tube. Once the reaction time was over, the tube was left in the heat block while it cooled to room temperature
  • Tube E - A 1.5 mL tube was used and 800 μL of water was added to the tube. The top of the tube was opened every 30 minutes, exposing the tube to the air. This tube was removed from the heat block when the reaction time was over.
  • Tube F - A 1.5 mL epitube was used. Only 400 μL of water was added to the BSA and HAuCl4. There was air present on top of the solution. Once the two hour reaction period was over, the tube was immediately removed from the heat block and was left to sit at room temperature.
  • Tube G - A 1.5 mL tube was used. 400 μL of water was added and there was space between the top of the tube and the top of the solution. At the end of the reaction time, the tube was left in the heat block while the temperature was slowly reduced.


Data

  • Tube A - Purple solution but then aggregated when out of heat to form purple fibers and a clear solution.
  • Tube B - Purple solution but then aggregated when out of heat to form large purple fibers and a clear solution.
  • Tube C - Small, lighter colored purple fibers were evenly scattered throughout a clear solution.
  • Tube D - Solution appeared purple and had small fibers floating throughout.
  • Tube E - Purple solution but then aggregated when out of heat to form a thin clump of purple fibers with lots of little, lighter colored fibers scattered throughout the clear solution.
  • Tube F - Clear solution with a big elongated clump of purple fibers.
  • Tube G - Clear solution with a big elongated clump of purple fibers.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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