User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 14: Line 14:
==Description==
==Description==
<u>Gold Nanoparticle Synthesis</u>
<u>Gold Nanoparticle Synthesis</u>
Five reaction mixtures were set up in epitubes.  All reaction mixtures contained 100 μL of BSA, 50 μL of HAuCl<sub>4</sub>, and 850 μL water.  The five tubes were heated at 80°C until a purple solution formed.  With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer. 


Tube 1 was
Five reaction mixtures were set up in epitubes.  All reaction mixtures contained 100 μL of BSA, 50 μL of HAuCl<sub>4</sub>, and 850 μL water.  The five tubes were heated in a heat block at 80°C until a purple solution formed.  With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer (1 M Tris, pH 6.8). 
 
* Tube 1 was removed from the heat block once a purple solution formed.
* Tube 2 was removed from the heat block and the contents were dumped into 1 mL of 1 M Tris (pH 6.8) at room temperature.
* Tube 3 was removed from the heat block and the contents were slowly transferred (using a Pasture pipette) to 1 mL room temperature of Tris buffer.
* Tube 4 was removed from the heat block and the contents were poured into 1 mL of Tris buffer at 80°C.
* Tube 5 was removed from the heat block and the contents were slowly added to 1 mL of buffer at 80°C.




Revision as of 12:45, 15 November 2011

Experimental Biological Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

To synthesize gold nanoparticles and to synthesize purple protein fibers.

Description

Gold Nanoparticle Synthesis

Five reaction mixtures were set up in epitubes. All reaction mixtures contained 100 μL of BSA, 50 μL of HAuCl4, and 850 μL water. The five tubes were heated in a heat block at 80°C until a purple solution formed. With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer (1 M Tris, pH 6.8).

  • Tube 1 was removed from the heat block once a purple solution formed.
  • Tube 2 was removed from the heat block and the contents were dumped into 1 mL of 1 M Tris (pH 6.8) at room temperature.
  • Tube 3 was removed from the heat block and the contents were slowly transferred (using a Pasture pipette) to 1 mL room temperature of Tris buffer.
  • Tube 4 was removed from the heat block and the contents were poured into 1 mL of Tris buffer at 80°C.
  • Tube 5 was removed from the heat block and the contents were slowly added to 1 mL of buffer at 80°C.


Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elizabeth_Ghias/Notebook/Experimental_Biological_Chemistry/2011/11/15&oldid=566409"