User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/15: Difference between revisions
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<u>Gold Nanoparticle Synthesis</u> | <u>Gold Nanoparticle Synthesis</u> | ||
Five reaction mixtures were set up in epitubes. All reaction mixtures contained 100 μL of BSA, 50 μL of HAuCl<sub>4</sub>, and 850 μL water. The five tubes were heated in a heat block at 80°C until a purple solution formed. With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer (1 M Tris, pH 6.8). | Five reaction mixtures were set up in epitubes. All reaction mixtures contained 100 μL of BSA (30 uM), 50 μL of HAuCl<sub>4</sub> (4.17 mM), and 850 μL water. The five tubes were heated in a heat block at 80°C until a purple solution formed. With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer (1 M Tris, pH 6.8). | ||
* Tube 1 was removed from the heat block once a purple solution formed. | * Tube 1 was removed from the heat block once a purple solution formed. | ||
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* Tube 5 was removed from the heat block and the contents were slowly added to 1 mL of buffer at 80°C. | * Tube 5 was removed from the heat block and the contents were slowly added to 1 mL of buffer at 80°C. | ||
<u>Protein Fiber Formation</u> | |||
Another reaction was set up to form purple fibers by mixing 1 mL of BSA, 1 mL of HAuCl<sub>4</sub> and 8.5 mL of water in a reaction tube. The reaction mixture was placed in an oven set to 78°C for two hours. | |||
==Data== | ==Data== | ||
* | * Tube 1 - Light purple solution | ||
* Tube 2 - The light purple solution became pink | |||
* Tube 3 - The light purple solution became a light pink | |||
* Tube 4 - The light purple solution became pink (darkest shade of pink) | |||
* Tube 5 - The light purple solution became light pink. | |||
* The reaction mixture that was expected to make protein fibers did not form any fibers. The solution was clear at the end of the reaction period. | |||
==Notes== | ==Notes== |
Revision as of 16:11, 3 December 2011
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo synthesize gold nanoparticles and to synthesize purple protein fibers. DescriptionGold Nanoparticle Synthesis Five reaction mixtures were set up in epitubes. All reaction mixtures contained 100 μL of BSA (30 uM), 50 μL of HAuCl4 (4.17 mM), and 850 μL water. The five tubes were heated in a heat block at 80°C until a purple solution formed. With the exception of tube 1, the contents of all five tubes were then added to a refolding buffer (1 M Tris, pH 6.8).
Data
NotesThis area is for any observations or conclusions that you would like to note.
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