User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/16: Difference between revisions
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==Objective== | ==Objective== | ||
To synthesize nanoparticles and investigate the effects of changing the concentration of the reactants, while keeping the molar ratio constant. | To synthesize nanoparticles and investigate the effects of changing the concentration of the reactants, while keeping the molar ratio constant, and to determine what solvents can be used to dissolve the purple fibers. | ||
==Description== | ==Description== | ||
<u>Nanoparticle Synthesis</u> | |||
Four reactions were performed. Three epitube reactions and one large glass test tube reactions were set up. | |||
The large test tube reaction consisted of 1 mL of 30uM BSA, 0.5 mL of 4.17 mM HAuCl<sub>4</sub>, and 8.5 mL water. The tube was placed in an oven set to 70°C for 3 hours. | |||
Epitube 1 - 100 uL BSA, 50 uL HAuCl<sub>4</sub>, and 850 uL water. Final Concentrations: | |||
Epitube 2 - 25 uL HAuCl<sub>4</sub>, 50 uL BSA, and 925 uL water in this order. Final Concentrations: | |||
Epitube 3 - 50 uL BSA, 25 uL HAuCl<sub>4</sub>, and 925 uL water in this order. Final Concentrations: | |||
<u>Dissolving Purple Fibers</u> | |||
The fibers were dissolved in four different solutions. | |||
Solution 1 - 1 mL of 1 M Tris | |||
Solution 2 - 1 mL of 6 M NaOH | |||
Solution 3 - 1 mL of DMSO | |||
Solution 4 - 1 mL of DMSO | |||
A small clump of fibers was added to solutions 1, 2, and 3. Due to the success of dissolving fibers in solution 3, a large amount of fibers was added to solution 4. | |||
==Data== | ==Data== | ||
* | * The large reaction tube had a dark vibrant purple color. | ||
* Epitubes 2 and 3 had the same color: a light pink-purple. Epitube 1 had a darker color than tubes 2 and 3. It was a light-medium purple color. | |||
* Fibers did not dissolve in solution 1. After 1 week, the fibers dissolved in solution 2. All of the fibers dissolved instantly in solution 2. A large amount of the fibers dissolved instantly in solution 4. After 1 week, the majority of the fibers had dissolved and the solution became a dark purple color. A small amount of tiny fibers could be seen floating around. | |||
==Notes== | ==Notes== | ||
Revision as of 13:43, 8 December 2011
 Experimental Biological Chemistry
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ObjectiveTo synthesize nanoparticles and investigate the effects of changing the concentration of the reactants, while keeping the molar ratio constant, and to determine what solvents can be used to dissolve the purple fibers. DescriptionNanoparticle Synthesis Four reactions were performed. Three epitube reactions and one large glass test tube reactions were set up. The large test tube reaction consisted of 1 mL of 30uM BSA, 0.5 mL of 4.17 mM HAuCl4, and 8.5 mL water. The tube was placed in an oven set to 70°C for 3 hours. Epitube 1 - 100 uL BSA, 50 uL HAuCl4, and 850 uL water. Final Concentrations: Epitube 2 - 25 uL HAuCl4, 50 uL BSA, and 925 uL water in this order. Final Concentrations: Epitube 3 - 50 uL BSA, 25 uL HAuCl4, and 925 uL water in this order. Final Concentrations:
Solution 1 - 1 mL of 1 M Tris Solution 2 - 1 mL of 6 M NaOH Solution 3 - 1 mL of DMSO Solution 4 - 1 mL of DMSO A small clump of fibers was added to solutions 1, 2, and 3. Due to the success of dissolving fibers in solution 3, a large amount of fibers was added to solution 4.
Data
NotesThis area is for any observations or conclusions that you would like to note.
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