User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/12/06
 Experimental Biological Chemistry
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ObjectiveTo compare the the 62.5 and 166.7 molar ratios for nanoparticle synthesis and attempt to produce purple fibers at the 67.6 ratio and a purple solutions at the 164.3 ratio. Description62.5 Molar Ratio Two 62.5 molar ratio reaction mixtures were prepared by mixing 100 uL of 30 uM BSA, 75 uL of 2.5 mM HAuCl4. and 825 uL water. Tube A was left in the heat block set to 80°C for 3 hours. Tube B was placed in the heat block and was taken out for twenty minutes every half an hour.
Two 166.7 molar ratio reaction mixtures were prepared by mixing 100 uL of 30 uM BSA, 200 uL of 2.5 mM HAuCl4. and 700 uL water. Tube C was left in the heat block set to 80°C for 3 hours. Tube D was placed in the heat block and was taken out for twenty minutes every half an hour.
Data
At the end of the reaction period tubes C and D were identical. Both tubes were then shaken. The fibers in tube C dissolved and a purple solution was created. The fibers in tube D did not dissolve. NotesThis area is for any observations or conclusions that you would like to note.
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