User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/12/07

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Experimental Biological Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

To investigate the effects of using an ice bath on nanoparticle synthesis.

Description

166.67 Molar Ratio

Two 166.67 molar ratio reaction mixtures were prepared by mixing 100 uL of 30 uM BSA, 200 uL of 2.5 mM HAuCl4. and 700 uL water.

Tube A was left in the heat block set to 80°C for 3 hours. Tube B was placed in the heat block and was taken out and placed in ice for ten minutes every half an hour.


62.5 Molar Ratio

Two 62.5 molar ratio reaction mixtures were prepared by mixing 100 uL of 30 uM BSA, 75 uL of 2.5 mM HAuCl4. and 825 uL water.

Tube C was left in the heat block set to 80°C for 3 hours. Tube D was placed in the heat block and was taken out and placed in ice for ten minutes every half an hour.


  • note - both 166.67 molar ratio tubes (A and B) were placed in the ice bath by accident. C and D were left in the heat block the entire time


Data

  • Tubes A and B were both clear at the end of the reaction period, while tubs C and D both had purple solutions.


Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elizabeth_Ghias/Notebook/Experimental_Biological_Chemistry/2011/12/07&oldid=571989"