User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/01/31: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 23: Line 23:
==Notes==
==Notes==
* The dye that will be used must either have a red shift or blue shift from the absorbance wavelength of the gold nanoparticles and must contain a succinimidyl group.
* The dye that will be used must either have a red shift or blue shift from the absorbance wavelength of the gold nanoparticles and must contain a succinimidyl group.
* The dye selected is 5(6)-Carboxynapthofluorescein, N-succinimidyl ester (C<sub>33</sub>H<sub>19</sub>NO<sub>9</sub>.  This dye is a long wavelength fluorophore that reacts with terminal and internal lysine residues at a pH of 8-9.  Its fluorescence is pH dependent.  It absorbs at 602 and emits at 672 nm.




Revision as of 11:15, 7 February 2012

Experimental Biological Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

To create stock solutions for the reactants used to produce gold nanoparticles and to decide which dye should be used for nanoparticle functionalization.

Description

  1. Make 40 mL of a 17.7 uM stock solution of BSA by mixing 0.0468 g BSA with 40 mL water.
  2. Make 20 mL of a 2.9 mM stock solution of HAuCl4 by mixing 0.0197 g HAuCl4 with 20 mL water.
  3. Selected a dye from Marker Gene Technologies Inc.


Data

No data was collected today

Notes

  • The dye that will be used must either have a red shift or blue shift from the absorbance wavelength of the gold nanoparticles and must contain a succinimidyl group.
  • The dye selected is 5(6)-Carboxynapthofluorescein, N-succinimidyl ester (C33H19NO9. This dye is a long wavelength fluorophore that reacts with terminal and internal lysine residues at a pH of 8-9. Its fluorescence is pH dependent. It absorbs at 602 and emits at 672 nm.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elizabeth_Ghias/Notebook/Experimental_Biological_Chemistry/2012/01/31&oldid=581621"