User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/02/08: Difference between revisions

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==Objective==
==Objective==
To make a Tris buffer, prepare the BSA control, and prepare additional gold nanoparticles solutions and purple fibers.


==Description==
==Description==
# The BSA control was prepared by mixing 1 mL BSA, 1 mL of 2.84 M HCl, and 8 mL water.  The reaction was placed in an oven set to 80°C for 2 hours.
# Gold nanoparticle solutions and purple fibers were prepared using the same procedure as February 1. 
# 250 mL of a 50 mM Tris buffer was prepared by mixing 3.512 mL 0.948 M HCl with 1.514 g Tris in a 250 mL volumetric flask. 


==Data==
==Data==
* Add data and results here...
* The pH of the Tris buffer was measured to be 8.73.


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]

Revision as of 11:30, 8 February 2012

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Objective

To make a Tris buffer, prepare the BSA control, and prepare additional gold nanoparticles solutions and purple fibers.

Description

  1. The BSA control was prepared by mixing 1 mL BSA, 1 mL of 2.84 M HCl, and 8 mL water. The reaction was placed in an oven set to 80°C for 2 hours.
  2. Gold nanoparticle solutions and purple fibers were prepared using the same procedure as February 1.
  3. 250 mL of a 50 mM Tris buffer was prepared by mixing 3.512 mL 0.948 M HCl with 1.514 g Tris in a 250 mL volumetric flask.

Data

  • The pH of the Tris buffer was measured to be 8.73.

Notes



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