User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/03/21: Difference between revisions

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==Data==
==Data==
* Add data and results here...
<u>Fluorescence Spectra After Centrifugation</u>
 
[[Image:21mar - fluorescence curve same sol.jpg]]
 
<u>Fluorescence Spectra After Changing the pH</u>
 
[[Image:21mar - fluorescence curve ph changedl.jpg]]
 
<u>UV-Vis Spectra After Changing the pH,/u.
 
[[Image:21mar - UV curve ph changed.jpg]]
 
[[Image:21mar - UV curve ph changed zoom.jpg]]


==Notes==
==Notes==

Revision as of 10:32, 10 April 2012

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Objective

To redo Fluorescence and UV-Vis Spectra, because the results obtained on 3/20 and 2/22 were not consistent. Spectra will be taken both after centrifugation and after pH changes.

Description

  1. The three dye solutions from the second dye attachment trial were centrifuged for five minutes at 13200 rpm.
  2. The fluorescence spectra were then taken for all three solutions with excitation at 600nm and emission between 620 and 800 nm.
  3. The pHs of the three solutions were then measured. The 166 ratio solution with dye had a pH of 8.13. The 70 ratio solution with dye had a pH of 7.62. The BSA/HCl solution with dye had an initial pH of 7.75.
  4. The pHs of the BSA/HCl and the 70 molar ratio solutions were raised by adding 50 mM Tris buffer. The new pH of the 70 molar ratio solution with dye was 8.07, while the new pH of the BSA/HCl solution with dye was 8.09.
  5. The three solutions were then centrifuged again at 13200 rpm for 5 minutes.
  6. Fluorescence spectra were taken for all three solutions with dye.


Data

Fluorescence Spectra After Centrifugation

Fluorescence Spectra After Changing the pH

UV-Vis Spectra After Changing the pH,/u.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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