User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/03/27: Difference between revisions

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==Description==
==Description==
# Add 50 mM tris buffer (pH = 8.73) to raise the pHs of the samples to a pH around 8.30.
# The samples containing fibers (166 molar ratio) were centrifuged for 5 minutes at 13,200 rpg.
# Fluorescence spectra were taken of the samples between 620 - 800 nm, with excitation at 600 nm.


==Data==
==Data==
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==Notes==
==Notes==
* When the pHs were initially measured, the values had changed from the week before
* When the pHs were initially measured, the values had changed from the week before
* The fluorescence spectra of the 70 molar ratio is now slightly higher than that of the 166 molar ratio




Revision as of 10:36, 27 March 2012

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Objective

To adjust the pHs of the solutions so that they are all equal and to retake UV-Vis and fluorescence spectra

Description

  1. Add 50 mM tris buffer (pH = 8.73) to raise the pHs of the samples to a pH around 8.30.
  2. The samples containing fibers (166 molar ratio) were centrifuged for 5 minutes at 13,200 rpg.
  3. Fluorescence spectra were taken of the samples between 620 - 800 nm, with excitation at 600 nm.


Data

  • Add data and results here...

Notes

  • When the pHs were initially measured, the values had changed from the week before
  • The fluorescence spectra of the 70 molar ratio is now slightly higher than that of the 166 molar ratio


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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