User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/03/27

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Objective

To adjust the pHs of all the dye solutions (both first and second trial) so that they are all equal and to retake UV-Vis and fluorescence spectra

Description

  1. Add 50 mM tris buffer (pH = 8.73) to raise the pHs of the samples to a pH around 8.30.
  2. The samples containing fibers (all dye samples) were centrifuged for 5 minutes at 13,200 rpg.
  3. Fluorescence spectra were taken of the samples between 620 - 800 nm, with excitation at 600 nm.
  4. Fluorescence spectra were also taken of the 70 molar ratio, 166 molar ratio, and the BSA/HCl reactions without dye.


Data

Fluorescence Spectra of Solutions from the Second Trial


Fluorescence Spectra of Solutions from the Initial Trial


Fluorescence Spectrum of Control solutions


UV Spectra of Solutions from the Second Trial


UV Spectra of Solutions from the Initial Trial


UV Spectrum of Control Solutions


Notes

  • When the pHs were initially measured, the values had changed from the week before
  • The fluorescence spectra of the 70 molar ratio with dye now has a slightly higher intensity than that of the 166 molar ratio with dye. The BSA/HCl dye mixture now has the lowest intensity


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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