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Objective
To adjust the pHs of all the dye solutions (both first and second trial) so that they are all equal and to retake UV-Vis and fluorescence spectra
Description
1. The initial pHs of the solutions from both the first and second trials were measured.
First Trial Initial pHs
- 70 molar ratio with dye pH = 8.12
- 166 molar ratio with dye pH = 8.43
- BSA/HCl with dye pH 8.30
Second Trial Initial pHs
- 70 molar ratio with dye pH = 8.00
- 166 molar ratio with dye pH = 8.13
- BSA/HCl with dye pH = 7.98
2. Add 50 mM tris buffer (pH = 8.73) to raise the pHs of the samples to a pH around 8.30.
First Trial pH Changes
- 70 molar ratio with dye: 10 drops of Tris were added to give a final pH of 8.30
Second Trial pH Changes
- 70 molar ratio with dye: 6 drops of Tris added, final pH = 8.29
- 166 molar ratio with dye: 3 drops of Tris added, final pH = 8.41
- BSA/HCl with dye: 7 drops of Tris added, final pH = 8.28
3. The samples containing fibers (all dye samples) were centrifuged for 5 minutes at 13,200 rpg.
4. Fluorescence spectra were taken of the samples between 620 - 800 nm, with excitation at 600 nm.
5. Fluorescence spectra were also taken of the 70 molar ratio, 166 molar ratio, and the BSA/HCl reactions without dye.
Data
Fluorescence Spectra of Solutions from the Second Trial
Fluorescence Spectra of Solutions from the Initial Trial
Fluorescence Spectrum of Control solutions
UV Spectra of Solutions from the Second Trial
UV Spectra of Solutions from the Initial Trial
UV Spectrum of Control Solutions
Notes
- When the pHs were initially measured, the values had changed from the week before. For example, the 166 molar ratio solution with dye from the second trial had a measured pH of 8.35 the previous week, but this week had a decreased pH of 8.13
- The fluorescence spectra of the 70 molar ratio with dye now has a slightly higher intensity than that of the 166 molar ratio with dye. The BSA/HCl dye mixture now has the lowest intensity
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Experimental Biological Chemistry
