To crosslink PVOH/glutaraldehyde and PVOH/PDMS/glutaraldehyde films, make a 1 g PVOH/glutaraldehyde film, make additional standards, and finish exchanging (from 9/26).
Crosslinking Glutaraldehyde Films
- Allow the PVOH film containing glutaraldehyde to dry completely.
- Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
- Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
- Heat solution to 70°C (temperature on hotplate should be about 160)
- Place film in solution for one hour.
- Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
- Heat HCl solution to 70°C
- Remove film from glutaraldehyde fixant and rinse with distilled water.
- Place film into HCl solution for 30 minutes.
- Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
- After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
- Use tweezers to remove the film from the sodium bicarbonate solution.
- Rinse film with deionized water and place in a beaker with distilled water until it is used.
- Add data and results here...
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