User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/10/24: Difference between revisions
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==Description== | ==Description== | ||
<u>Crosslinking Glutaraldehyde Films</u> | |||
# Allow the PVOH film containing glutaraldehyde to dry completely. | |||
# Prepare a solution of 100 mL H<sub>2</sub>O, 2 mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20 g Na<sub>2</sub>SO<sub>4</sub> in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
# Heat solution to 70°C (temperature on hotplate should be about 160) | |||
# Place film in solution for one hour. | |||
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
# Heat HCl solution to 70°C | |||
# Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
# Place film into HCl solution for 30 minutes. | |||
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
# Use tweezers to remove the film from the sodium bicarbonate solution. | |||
# Rinse film with deionized water and dry film on paper towel. | |||
# Weigh film and record mass. | |||
Revision as of 08:28, 24 October 2012
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo crosslink the PVOH/glutaraldehyde film (made on Monday), make a new prophyrin film with 2 drops of HCl, to make additional glutaraldehyde fixant, and to prepare a new PVOH/Na-MT/glutaraldehyde film.
DescriptionCrosslinking Glutaraldehyde Films
Data
NotesThis area is for any observations or conclusions that you would like to note.
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