User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/10/24

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(Autocreate 2012/10/24 Entry for User:Elizabeth_Ghias/Notebook/Experimental_Chemistry)
Current revision (09:25, 7 November 2012) (view source)
 
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==Objective==
==Objective==
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To crosslink the PVOH/glutaraldehyde film (made on Monday), make a new prophyrin film with 2 drops of HCl, to make additional glutaraldehyde fixant, and to prepare a new PVOH/Na-MT/glutaraldehyde film. 
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==Description==
==Description==
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<u>Crosslinking Glutaraldehyde Films</u>
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# Allow the PVOH film containing glutaraldehyde to dry completely.
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# Prepare a solution of 100 mL H<sub>2</sub>O, 2 mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20 g Na<sub>2</sub>SO<sub>4</sub> in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) 
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# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
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# Heat solution to 70°C (temperature on hotplate should be about 160)
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# Place film in solution for one hour. 
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# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker.  Place a stir bar and an inverted buchner funnel into beaker. 
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# Heat HCl solution to 70°C
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# Remove film from glutaraldehyde fixant and rinse with distilled water.
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# Place film into HCl solution for 30 minutes.
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# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
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# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
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# Use tweezers to remove the film from the sodium bicarbonate solution. 
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# Rinse film with deionized water and dry film on paper towel. 
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# Weigh film and record mass.
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<u>Porphyrin Film Preparation</u>
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# 1 g of PVOH was dissolved in 7 mL H<sub>2</sub>O on a heated hot plate.
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# Once the PVOH dissolved, 1 mL of glutaraldehyde, 1 mL of porphyrin, and 2-3 drops of HCl was added.
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# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.
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<u>Na-MT Film Preparation</u>
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# 1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H<sub>2</sub>O on a heated hot plate.
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# Once the PVOH dissolved, 1 mL of glutaraldehyde was added.
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# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.
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==Data==
==Data==
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* Add data and results here...
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No data was collected today.
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==Notes==
==Notes==

Current revision

Experimental Chemistry Main project page
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Objective

To crosslink the PVOH/glutaraldehyde film (made on Monday), make a new prophyrin film with 2 drops of HCl, to make additional glutaraldehyde fixant, and to prepare a new PVOH/Na-MT/glutaraldehyde film.


Description

Crosslinking Glutaraldehyde Films

  1. Allow the PVOH film containing glutaraldehyde to dry completely.
  2. Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
  3. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
  4. Heat solution to 70°C (temperature on hotplate should be about 160)
  5. Place film in solution for one hour.
  6. Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
  7. Heat HCl solution to 70°C
  8. Remove film from glutaraldehyde fixant and rinse with distilled water.
  9. Place film into HCl solution for 30 minutes.
  10. Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
  11. After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
  12. Use tweezers to remove the film from the sodium bicarbonate solution.
  13. Rinse film with deionized water and dry film on paper towel.
  14. Weigh film and record mass.


Porphyrin Film Preparation

  1. 1 g of PVOH was dissolved in 7 mL H2O on a heated hot plate.
  2. Once the PVOH dissolved, 1 mL of glutaraldehyde, 1 mL of porphyrin, and 2-3 drops of HCl was added.
  3. The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.


Na-MT Film Preparation

  1. 1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H2O on a heated hot plate.
  2. Once the PVOH dissolved, 1 mL of glutaraldehyde was added.
  3. The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.


Data

No data was collected today.


Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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