User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/11/14

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Description)
Line 11: Line 11:
==Objective==
==Objective==
-
Prepare 3 PVOH/glutaraldehyde films, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na<sub>2</sub>SO<sub>4</sub>
+
Prepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na<sub>2</sub>SO<sub>4</sub>
==Description==
==Description==
-
<u>PVOH/glutaraldehyde film preparation</u>
+
<u>PVOH/Glutaraldehyde Film Preparation</u>
 +
 
 +
# 1 g PVOH was dissolved in 7 mL of water.
 +
# Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added.
 +
# The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Friday.
 +
 
 +
 
 +
<u>PVOH/Na-MT/Glutaraldehyde Film Preparation</u>
 +
 
 +
# 1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H<sub>2</sub>O on a heated hot plate.
 +
# Once the PVOH dissolved, 1 mL of glutaraldehyde was added.
 +
# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.
<u>PVOH/Dye film preparation</u>
<u>PVOH/Dye film preparation</u>
-
#1gPVOH
+
# Place 1 g PVOH in a small beaker
-
#Dissolve in 7mL of 0.1M NaHCO3/Na2CO3 buffer (pH=10) to deprotonate  
+
# Dissolve in 7 mL of 0.1M NaHCO3/Na2CO3 buffer (pH=10) to deprotonate  
-
#Heat and Stir to dissolve
+
# Heat and stir to dissolve
-
#Add 1mg of dye
+
# Add 1 mg of dye (5-(4,6-Dichlorotiazinul Aminofluorescein)
-
#cover in foil
+
# Stir for 1 hr
-
#stir for 1 hr
+
# Pour into dish
-
#pour into dish
+
 
 +
 
 +
Z
==Data==
==Data==
-
* Add data and results here...
 
 +
* No data was collected today
 +
==Notes==
==Notes==
-
This area is for any observations or conclusions that you would like to note.
 
 +
* The PVOH/dye solution turned a bright fluorescent yellow immediately as the dye was added.
-
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Course]]

Revision as of 11:09, 14 November 2012

Experimental Chemistry Main project page
Previous entry      Next entry


Objective

Prepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na2SO4

Description

PVOH/Glutaraldehyde Film Preparation

  1. 1 g PVOH was dissolved in 7 mL of water.
  2. Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added.
  3. The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Friday.


PVOH/Na-MT/Glutaraldehyde Film Preparation

  1. 1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H2O on a heated hot plate.
  2. Once the PVOH dissolved, 1 mL of glutaraldehyde was added.
  3. The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday.


PVOH/Dye film preparation

  1. Place 1 g PVOH in a small beaker
  2. Dissolve in 7 mL of 0.1M NaHCO3/Na2CO3 buffer (pH=10) to deprotonate
  3. Heat and stir to dissolve
  4. Add 1 mg of dye (5-(4,6-Dichlorotiazinul Aminofluorescein)
  5. Stir for 1 hr
  6. Pour into dish


Z

Data

  • No data was collected today

Notes

  • The PVOH/dye solution turned a bright fluorescent yellow immediately as the dye was added.



Personal tools