User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/11/19

Objective

To determine the swelling of the PVOH/glutaraldehyde films, prepare another PVOH/glutaraldehyde/porphyrin/HCl film, conduct DSC, conduct FTIR,

Description

Crosslinking Glutaraldehyde Films

1. Allow the PVOH film containing glutaraldehyde to dry completely.
2. Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
3. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
4. Heat solution to 70°C (temperature on hotplate should be about 160)
5. Place film in solution for one hour.
6. Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
7. Heat HCl solution to 70°C
8. Remove film from glutaraldehyde fixant and rinse with distilled water.
9. Place film into HCl solution for 30 minutes.
10. Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
11. After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
12. Use tweezers to remove the film from the sodium bicarbonate solution.
13. Rinse film with deionized water and dry film on paper towel.

Swelling

1. Determine the mass of the PVOH/glutaraldehyde film (has not been crosslinked).
2. Place the film in a 250 mL beaker filled with 30 mL water.
3. Cover beaker with parafilm to prevent evaporation.
4. Allow film to soak for ____________
5. Measure the volume of water remaining and the mass of the film.

Porphyrin Film Preparation

1. 1 g of PVOH was dissolved in 7 mL H₂O on a heated hot plate.
2. Once the PVOH dissolved, 1 mL of glutaraldehyde, 1 mL of porphyrin, and 2-3 drops of HCl was added.
3. The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate for 1 week.

DCS

FTIR

Data

• Add data and results here...

Notes

• After break, do Na+ and Cu2+ absorption with crosslinked PVOH/glutaraldehyde films,

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