User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/11/19: Difference between revisions
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==Objective== | ==Objective== | ||
To determine the swelling of the PVOH/glutaraldehyde films, prepare another PVOH/glutaraldehyde/porphyrin/HCl film, conduct DSC, conduct FTIR, | |||
==Description== | ==Description== | ||
Crosslinking Glutaraldehyde Films | |||
#Allow the PVOH film containing glutaraldehyde to dry completely. | |||
#Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
#Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
#Heat solution to 70°C (temperature on hotplate should be about 160) | |||
#Place film in solution for one hour. | |||
#Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
#Heat HCl solution to 70°C | |||
#Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
#Place film into HCl solution for 30 minutes. | |||
#Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
#After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
#Use tweezers to remove the film from the sodium bicarbonate solution. | |||
#Rinse film with deionized water and dry film on paper towel. | |||
<u>Swelling</u> | |||
# One of the PVOH/glutaraldehyde films that had been crosslinked on 11/16 was removed from water. | |||
# The mass of the film was measured. | |||
# The film was dried in an oven set to 90°C for about 30 minutes and then was left to air dry for 1 week. | |||
<u>Porphyrin Film Preparation</u> | |||
* 3 porphyrin films were prepared. Two of the films contained the HCl seen in step 2. The HCl portion of step 2 was omitted. | |||
# 1 g of PVOH was dissolved in 7 mL H<sub>2</sub>O on a heated hot plate. | |||
# Once the PVOH dissolved, 1 mL of glutaraldehyde, 1 mL of porphyrin, and 2-3 drops of HCl was added. | |||
# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate for 1 week. | |||
<u>DCS</u> | |||
<u>FTIR</u> | |||
==Data== | ==Data== | ||
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==Notes== | ==Notes== | ||
* After break, do Na+ and Cu2+ absorption with crosslinked PVOH/glutaraldehyde films, | |||
Revision as of 13:12, 19 November 2012
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ObjectiveTo determine the swelling of the PVOH/glutaraldehyde films, prepare another PVOH/glutaraldehyde/porphyrin/HCl film, conduct DSC, conduct FTIR,
DescriptionCrosslinking Glutaraldehyde Films
Swelling
Porphyrin Film Preparation
DCS
Data
Notes
|