User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/01: Difference between revisions
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==Description== | ==Description== | ||
<u>Analyze FTIR</u> | |||
* By looking at the FTIR spectra of our films, we determined that the quaternary ammonium salt was present in our film. Therefore we will move on to bacteria testing. | |||
<u>LB Broth Preparation</u> | <u>LB Broth Preparation</u> | ||
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<u>Hydrogel Autoclaving</u> | <u>Hydrogel Autoclaving</u> | ||
# Allow the PVOH film containing glutaraldehyde to dry completely. | |||
# Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
# Heat solution to 70°C (temperature on hotplate should be about 160) | |||
# Place film in solution for one hour. | |||
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
# Heat HCl solution to 70°C | |||
# Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
# Place film into HCl solution for 30 minutes. | |||
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
# Use tweezers to remove the film from the sodium bicarbonate solution. | |||
# Rinse film with deionized water. | |||
# The films were then allowed to dry over the weekend. | |||
Revision as of 13:43, 8 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectivePrepare LB solutions for bacteria testing next week and crosslink the hydrogels that contain glutaraldehyde. DescriptionAnalyze FTIR
DataNo data was collected today. NotesThis area is for any observations or conclusions that you would like to note.
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