User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/01

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 14: Line 14:
==Description==
==Description==
 +
 +
<u>Analyze FTIR</u>
 +
 +
* By looking at the FTIR spectra of our films, we determined that the quaternary ammonium salt was present in our film.  Therefore we will move on to bacteria testing.
 +
<u>LB Broth Preparation</u>
<u>LB Broth Preparation</u>
Line 24: Line 29:
<u>Hydrogel Autoclaving</u>
<u>Hydrogel Autoclaving</u>
 +
# Allow the PVOH film containing glutaraldehyde to dry completely.
 +
# Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
 +
# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
 +
# Heat solution to 70°C (temperature on hotplate should be about 160)
 +
# Place film in solution for one hour.
 +
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
 +
# Heat HCl solution to 70°C
 +
# Remove film from glutaraldehyde fixant and rinse with distilled water.
 +
# Place film into HCl solution for 30 minutes.
 +
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
 +
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
 +
# Use tweezers to remove the film from the sodium bicarbonate solution.
 +
# Rinse film with deionized water.
 +
# The films were then allowed to dry over the weekend.

Revision as of 16:43, 8 March 2013

Experimental Chemistry Main project page
Previous entry      Next entry


Objective

Prepare LB solutions for bacteria testing next week and crosslink the hydrogels that contain glutaraldehyde.

Description

Analyze FTIR

  • By looking at the FTIR spectra of our films, we determined that the quaternary ammonium salt was present in our film. Therefore we will move on to bacteria testing.


LB Broth Preparation

  1. Make one solution of 1.875 g LB broth in 75 mL water and make 8 solutions of 1.25 g LB broth in 50 mL water
  2. Cap all with foil
  3. Autoclave solutions for one hour


Hydrogel Autoclaving

  1. Allow the PVOH film containing glutaraldehyde to dry completely.
  2. Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
  3. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
  4. Heat solution to 70°C (temperature on hotplate should be about 160)
  5. Place film in solution for one hour.
  6. Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
  7. Heat HCl solution to 70°C
  8. Remove film from glutaraldehyde fixant and rinse with distilled water.
  9. Place film into HCl solution for 30 minutes.
  10. Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
  11. After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
  12. Use tweezers to remove the film from the sodium bicarbonate solution.
  13. Rinse film with deionized water.
  14. The films were then allowed to dry over the weekend.


Data

No data was collected today.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools