User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04

Experimental Chemistry

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Objective To prepare agar plates and to prepare the starter culture of bacteria. Description Starter Culture Preparation Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) Place the tubes on a shaker at 37°C overnight. Agar Plate Preparation Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. The solution was autoclaved for one hour Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. This made about 20 agar plates The plates were left in the fridge to set Data No data was collected today Notes Because of snow day this week, bacteria were left on shaker until Thursday. To Do List this Week Make additional quaternary ammonium salt films (wed or tues, Noah) Crosslink quaternary ammonium salt films (thurs/fri) Split the bacteria from the starter culture between the flasks for testing. Measure UV Vis of bacteria in starter culture before adding films Put films (cut in half) in flasks, then take periodic UV vis throughout the test. DSC data analysis