User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04: Difference between revisions
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==Description== | ==Description== | ||
<u>Starter Culture Preparation</u> | |||
# Make one solution of 1.875 g LB broth in 75 mL water and make 8 solutions of 1.25 g LB broth in 50 mL water | |||
# Cap all with foil | |||
# Autoclave solutions for one hour | |||
# For the 75 mL solution, take out 4 mL broth and put into 4 test tubes | |||
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | |||
# Place the tubes on a shaker at 37°C overnight. | |||
<u>Agar Plate Preparation</u> | <u>Agar Plate Preparation</u> | ||
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# The plates were autoclaved for one hour | # The plates were autoclaved for one hour | ||
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | # Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | ||
#* This made 20 agar plates | #* This made about 20 agar plates | ||
# The plates were left in the fridge to set | |||
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==Notes== | ==Notes== | ||
* Because of snow day this week, bacteria were left on shaker until Thursday. | |||
<u>To Do List this Week</u> | <u>To Do List this Week</u> |
Revision as of 13:31, 8 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo prepare agar plates and to prepare the starter culture of bacteria. DescriptionStarter Culture Preparation
Agar Plate Preparation
DataNo data was collected today Notes
To Do List this Week
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