User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04: Difference between revisions

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<u>Starter Culture Preparation</u>
<u>Starter Culture Preparation</u>


# Make one solution of 1.875 g LB broth in 75 mL water and make 8 solutions of 1.25 g LB broth in 50 mL water
# Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes
# Cap all with foil
# Autoclave solutions for one hour
# For the 75 mL solution, take out 4 mL broth and put into 4 test tubes
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)
# Place the tubes on a shaker at 37°C overnight.
# Place the tubes on a shaker at 37°C overnight.
Line 29: Line 26:


# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.
# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.
# The plates were autoclaved for one hour
# The solution was autoclaved for one hour
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.  
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.  
#* This made about 20 agar plates
#* This made about 20 agar plates
# The plates were left in the fridge to set
# The plates were left in the fridge to set


==Data==
==Data==

Revision as of 19:13, 26 March 2013

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Objective

To prepare agar plates and to prepare the starter culture of bacteria.

Description

Starter Culture Preparation

  1. Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes
  2. To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)
  3. Place the tubes on a shaker at 37°C overnight.


Agar Plate Preparation

  1. Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.
  2. The solution was autoclaved for one hour
  3. Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.
    • This made about 20 agar plates
  4. The plates were left in the fridge to set

Data

No data was collected today

Notes

  • Because of snow day this week, bacteria were left on shaker until Thursday.

To Do List this Week

  • Make additional quaternary ammonium salt films (wed or tues, Noah)
  • Crosslink quaternary ammonium salt films (thurs/fri)
  • Split the bacteria from the starter culture between the flasks for testing.
  • Measure UV Vis of bacteria in starter culture before adding films
  • Put films (cut in half) in flasks, then take periodic UV vis throughout the test.
  • DSC data analysis



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