User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04 - Revision history

Klare Lazor: /* Description */

2013-03-27T02:13:41Z

Description

←Older revision		Revision as of 02:13, 27 March 2013
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	#* This made about 20 agar plates		#* This made about 20 agar plates
	# The plates were left in the fridge to set		# The plates were left in the fridge to set
-

	==Data==		==Data==

Elizabeth Ghias at 20:41, 8 March 2013

2013-03-08T20:41:15Z

←Older revision		Revision as of 20:41, 8 March 2013
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	<u>Starter Culture Preparation</u>		<u>Starter Culture Preparation</u>

-	# ~~Make one solution~~ of ~~1.875 g LB~~ broth in 75 mL ~~water and make 8 solutions of 1.25 g~~ LB broth ~~in 50 mL water~~	+	# Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes
-	~~# Cap all with foil~~	+
-	~~# Autoclave solutions for one hour~~	+
-	~~# For the 75 mL~~ solution~~, take out 4 mL broth~~ and put into 4 test tubes	+
	# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)		# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)
	# Place the tubes on a shaker at 37°C overnight.		# Place the tubes on a shaker at 37°C overnight.
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	# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.		# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.
-	# The ~~plates were~~ autoclaved for one hour	+	# The solution was autoclaved for one hour
	# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.		# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.
	#* This made about 20 agar plates		#* This made about 20 agar plates

Elizabeth Ghias at 20:31, 8 March 2013

2013-03-08T20:31:55Z

←Older revision		Revision as of 20:31, 8 March 2013
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	==Description==		==Description==
		+
		+	<u>Starter Culture Preparation</u>
		+
		+	# Make one solution of 1.875 g LB broth in 75 mL water and make 8 solutions of 1.25 g LB broth in 50 mL water
		+	# Cap all with foil
		+	# Autoclave solutions for one hour
		+	# For the 75 mL solution, take out 4 mL broth and put into 4 test tubes
		+	# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame)
		+	# Place the tubes on a shaker at 37°C overnight.
		+
		+

	<u>Agar Plate Preparation</u>		<u>Agar Plate Preparation</u>
Line 20:		Line 31:
	# The plates were autoclaved for one hour		# The plates were autoclaved for one hour
	# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.		# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.
-	#* This made 20 agar plates	+	#* This made about 20 agar plates
		+	# The plates were left in the fridge to set


Line 28:		Line 40:

	==Notes==		==Notes==
		+
		+	* Because of snow day this week, bacteria were left on shaker until Thursday.

	<u>To Do List this Week</u>		<u>To Do List this Week</u>

Elizabeth Ghias at 19:26, 4 March 2013

2013-03-04T19:26:29Z

←Older revision		Revision as of 19:26, 4 March 2013
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	* Measure UV Vis of bacteria in starter culture before adding films		* Measure UV Vis of bacteria in starter culture before adding films
	* Put films (cut in half) in flasks, then take periodic UV vis throughout the test.		* Put films (cut in half) in flasks, then take periodic UV vis throughout the test.
		+	* DSC data analysis

Elizabeth Ghias at 19:24, 4 March 2013

2013-03-04T19:24:53Z

←Older revision		Revision as of 19:24, 4 March 2013
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-	==Data==y	+	==Data==

	No data was collected today		No data was collected today

Elizabeth Ghias at 19:24, 4 March 2013

2013-03-04T19:24:33Z

←Older revision		Revision as of 19:24, 4 March 2013
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	==Objective==		==Objective==
		+
		+	To prepare agar plates and to prepare the starter culture of bacteria.

	==Description==		==Description==

-	~~==Data==~~	+	<u>Agar Plate Preparation</u>
-	* Add data and results here...	+

-	~~==Notes==~~	+	# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water.
-	~~This area is~~ for ~~any observations or conclusions that you would like to note~~.	+	# The plates were autoclaved for one hour
		+	# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes.
		+	#* This made 20 agar plates


-	Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.	+	==Data==y
		+
		+	No data was collected today
		+
		+	==Notes==

-	~~[[Category:Course]]~~	+	<u>To Do List this Week</u>
-	~~[[Category:Miscellaneous]]~~	+	* Make additional quaternary ammonium salt films (wed or tues, Noah)
		+	* Crosslink quaternary ammonium salt films (thurs/fri)
		+	* Split the bacteria from the starter culture between the flasks for testing.
		+	* Measure UV Vis of bacteria in starter culture before adding films
		+	* Put films (cut in half) in flasks, then take periodic UV vis throughout the test.

Elizabeth Ghias: Autocreate 2013/03/04 Entry for User:Elizabeth_Ghias/Notebook/Experimental_Chemistry

2013-03-04T18:48:41Z

Autocreate 2013/03/04 Entry for User:Elizabeth_Ghias/Notebook/Experimental_Chemistry

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|


==Objective==

==Description==

==Data==
* Add data and results here...

==Notes==
This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

[[Category:Course]]
[[Category:Miscellaneous]]


|}

__NOTOC__