User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/07: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<u>Agar Plate Preparation</u> | <u>Agar Plate Preparation</u> | ||
* 50 mL of bacteria was pipetted onto each plate | |||
* The inoculation loop was placed in ethanol and then placed through a flame. | |||
* The loop was then used to spread around the bacteria. | |||
* Samples were then added to the bacteria (see above). For the samples containing half of a film, the flattest portions of the films were used. Tape was used to try and push the films flat against the surface of the plate | |||
* The plates were then covered and placed in an oven set to 37°C overnight. | |||
* The films will be visualized on Friday. | |||
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==Notes== | ==Notes== | ||
* Sterile techniques were used for all of these tests (flame) | |||
* The tape was ineffective at holding the film against the plate b/c it could not stick to the plate. It also blocked portions of the sample and made visualization more difficult. | |||
Latest revision as of 22:32, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectiveTo inoculate the agar plates and the previously autoclaved LB broth for bacterial testing DescriptionLB broth large flask testing
DataUV Vis Data
Notes
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