User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/08

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(Autocreate 2013/03/08 Entry for User:Elizabeth_Ghias/Notebook/Experimental_Chemistry)
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==Objective==
==Objective==
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To modify PVOH with quaternary ammonium salt and to analyze DSC data from February and the agar plates prepared yesterday.
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==Description==
==Description==
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<u>(S)-(-)-(3-Chloro-2-hydroxypropyl)trimethylammonium chloride Reaction</u>
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# Measure out 4 samples of 2 g PVOH (MW = 22,000)
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# Measure out 0.85779 g (S)-(-)-(3-Chloro-2-hydroxypropyl)trimethylammonium chloride. Place in 5 mL 0.2 M NaOH prepared last class)
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#* Actual amount used = 0.8581 g
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# Transfer 0.5 mL, 0.05 mL, and 0.005 mL into 4.5 mL, 4.95 mL and 4.995 mL 0.2 M NaOH, respectively (this means the 10% film will be slightly less than 10% but we can still calculate what percentage)→ using 10 mL total volume b/c were doing 2 gram scale.  For 1 g scale use 5 mL.
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# Place the 4 samples of PVOH in 5 mL
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# Allow to soak in the NaOH for 30 minutes
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# Add 1 sample PVOH to one of the ammonium chloride samples and allow them to mix for 1 hour
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# If the PVOH is still soluble in NaOH, add acetone to precipitate it out. (keep water to a minimum)
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# Then do a quick wash with ice water to get precipitate out extra ions
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<u>Agar Plate Analysis</u>
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* The agar plates were visualized to compare the relative amount of bacteria growth that occurred overnight.
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<u>DSC Data Analysis</u>
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* For DSC data analysis, glass transitions were found and any peaks present were integrated.
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==Data==
==Data==
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* Add data and results here...
 
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==Notes==
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<u>Agar Plate Visualization</u>
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This area is for any observations or conclusions that you would like to note.
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* For the agar plates, the only two plates that were observed to limit bacteria growth were plates 7 and 8, which both contained the highest amount of quaternary ammonium salt.
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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<u>DSC Results</u>
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[[Category:Course]]
 
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[[Category:Miscellaneous]]
 
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==Notes==

Revision as of 14:26, 8 March 2013

Experimental Chemistry Main project page
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Objective

To modify PVOH with quaternary ammonium salt and to analyze DSC data from February and the agar plates prepared yesterday.


Description

(S)-(-)-(3-Chloro-2-hydroxypropyl)trimethylammonium chloride Reaction

  1. Measure out 4 samples of 2 g PVOH (MW = 22,000)
  2. Measure out 0.85779 g (S)-(-)-(3-Chloro-2-hydroxypropyl)trimethylammonium chloride. Place in 5 mL 0.2 M NaOH prepared last class)
    • Actual amount used = 0.8581 g
  3. Transfer 0.5 mL, 0.05 mL, and 0.005 mL into 4.5 mL, 4.95 mL and 4.995 mL 0.2 M NaOH, respectively (this means the 10% film will be slightly less than 10% but we can still calculate what percentage)→ using 10 mL total volume b/c were doing 2 gram scale. For 1 g scale use 5 mL.
  4. Place the 4 samples of PVOH in 5 mL
  5. Allow to soak in the NaOH for 30 minutes
  6. Add 1 sample PVOH to one of the ammonium chloride samples and allow them to mix for 1 hour
  7. If the PVOH is still soluble in NaOH, add acetone to precipitate it out. (keep water to a minimum)
  8. Then do a quick wash with ice water to get precipitate out extra ions


Agar Plate Analysis

  • The agar plates were visualized to compare the relative amount of bacteria growth that occurred overnight.


DSC Data Analysis

  • For DSC data analysis, glass transitions were found and any peaks present were integrated.


Data

Agar Plate Visualization

  • For the agar plates, the only two plates that were observed to limit bacteria growth were plates 7 and 8, which both contained the highest amount of quaternary ammonium salt.

DSC Results


Notes


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