User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
Added the calculated amounts of protein to the hydrogels + NHS-azide and plated the bacteria for the QAS test | |||
==Description== | ==Description== | ||
= | <u>Protein Addition</u> | ||
# 10 mL of phosphate buffer (ph = 7.6) was placed in a vial (repeat 4 times) | |||
# A hydrogel + NHS-azide and a stir bar was added to each vial | |||
# The corresponding amount of protein (see yesterday for calculations) was added to each vial | |||
# The vials were allowed to stir overnight | |||
<u>Bacterial Testing</u> | |||
# Each of the QAS films and a PVOH control were soaked in water and cut into small circles. | |||
# 50 uL of bacteria was plated on each agar plate. Sterile techniques were used | |||
# The film sample was placed on the agar plate | |||
# 5 uL of bacteria was added to each circle/sample | |||
# The plates were placed in the incubator overnight. | |||
==Data== | |||
No data was collected today | |||
==Notes== | |||
* The QAS films used for testing were being reused from the last bacterial test. Not all the bacteria could be washed away by ethanol. | |||
Latest revision as of 22:39, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectiveAdded the calculated amounts of protein to the hydrogels + NHS-azide and plated the bacteria for the QAS test DescriptionProtein Addition
DataNo data was collected today Notes
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