User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/19

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<u>Determining the Concentration of the DNA stock</u>
<u>Determining the Concentration of the DNA stock</u>
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* We want to add 200 ng DNA to each sample for activity testing.
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* the absorbance and concentration of DNA are linearly related according to the equation:
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unknown [DNA]/A<sub>260</sub> = 50 ug/ml/1.0
==Data==
==Data==

Revision as of 15:04, 3 May 2013

Experimental Chemistry Main project page
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Objective

To prepare a tris buffer for the DNase activity testing and to determine the concentration of DNA in the DNA stock to be used for activity testing.

Description

Determining the Concentration of the DNA stock

  • We want to add 200 ng DNA to each sample for activity testing.
  • the absorbance and concentration of DNA are linearly related according to the equation:

unknown [DNA]/A260 = 50 ug/ml/1.0

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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