User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/22: Difference between revisions

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==Objective==
==Objective==
To test the ability of the DNase I (attached to the hydrogels) to cleave DNA


==Description==
==Description==
# In addition to the 5 samples of hydrogels with DNA, a sample of hydrogel without DNase and a sample of DNase I on its own were prepared as controls.  The amount of DNase used for the control was equivalent to the amount of enzyme in the 500 uL hydrogel
# 1 uL of DNA from Chem 571 last year was added to each sample
# The DNA and DNase were mixed together for 3-4 hours. 
# The hydrogels were then removed from the samples and the liquid was then frozen using liquid nitrogen.  The sampes were then lyophilized overnight. 


==Data==
==Data==
* Add data and results here...
 
No data was collected today.


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
* This reaction was carried out at room temperature, not 37°C, which may have reduced or prevented enzymatic activity
[[Category:Miscellaneous]]




Revision as of 11:54, 3 May 2013

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Objective

To test the ability of the DNase I (attached to the hydrogels) to cleave DNA

Description

  1. In addition to the 5 samples of hydrogels with DNA, a sample of hydrogel without DNase and a sample of DNase I on its own were prepared as controls. The amount of DNase used for the control was equivalent to the amount of enzyme in the 500 uL hydrogel
  2. 1 uL of DNA from Chem 571 last year was added to each sample
  3. The DNA and DNase were mixed together for 3-4 hours.
  4. The hydrogels were then removed from the samples and the liquid was then frozen using liquid nitrogen. The sampes were then lyophilized overnight.

Data

No data was collected today.

Notes

  • This reaction was carried out at room temperature, not 37°C, which may have reduced or prevented enzymatic activity



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