User:Emilienne

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Gene Functions and Re-engineering Ideas)
(Gene Functions and Re-engineering Ideas)
Line 51: Line 51:
|--
|--
| PI
| PI
-
| Phage Secretion: C-terminals of P1 and P11 interact with N-terminal of P4 to form a complex through which mature phage is secreted.
+
| *Phage Secretion: C-terminals of P1 and P11 interact with N-terminal of P4 to form a complex through which mature phage is secreted.
|  
|  
|--
|--
 +
|PII
 +
|*Replication: Initiates genome replication by nicking the double stranded phage DNA
 +
|Without P2, could the bacteria still make the phage gene products without replicating the phage DNA? If we remove P2 (and P5), could we use this virus to insert other genes into the bacteria? NOTE: Altering the second half of P2 will affect P10.
 +
|--
 +
|PIII
 +
|*Rounded Tip of Filament: First protein to interact with the E. coli host pilus during infection and last point of contact as phages bud from bacteria. without P3, phage cannot fully escape host.
 +
|We might change P3 such that the phage was only capable of infecting a different host other than E. coli, if the need arose.
 +
|--
 +
|PIV
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
|
|
|
|

Revision as of 00:51, 14 February 2007

Contents

Student Registration/Questionnaire

Please copy the source code from this page to your user page (click on the Image:EditButton.jpg button above, and copy everything inside the box to your user page). Fill in the answers and print out a copy for next time. You do not need to keep the information on your user page once you've printed it out.

20.109 Spring 2007

Last Name

Repak

First Name

Emilienne

Preferred name

Course/Minor

Course 20, minor in French, strong interest in neuroscience

Year of Graduation

2009

Telephone #

(267)566-1418

Email

emilie7 at mit dot edu

Have you taken

7.05/5.07 (Biochemistry) currently taking
7.06 (Cell Biology) no
7.02 (General Biology Lab) no
5.310 (General Chemistry Lab) no

Do you have any experience culturing cells (mammalian, yeast or microbial)?
No

Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
No

Please briefly describe any previous laboratory experience

UROP in Fee Lab in McGovern Institute, BCS. February 06- present. I do brain surgery on songbirds (lesions, tracers, and electrophysiology), perform profusions, histology, immunochemistry, and microscopy.

Anything else you would like us to know?

Optional: As mentioned in lecture on 02.06.07, we would like you to consider how, as a biological engineer you might test the superstition "Spit on a bat before using it for the 1st time to make it lucky."

I would perform a double blind experiment in which both athletes testing bats and evaluators scoring performance of the bats do not know which bats have been spit upon. One could then statistically analyze the data from bats that had and had not been spit upon to see if bats with spit on them were any luckier. If, as I suspect, they are not, then I have proven that I do not need to go into molecular analysis of the spit to try to find the elements which contribute to the luck.

M13 Genome Engineering

Gene Functions and Re-engineering Ideas

GENE FUNCTION POTENTIAL MODIFICATION(S)
PI *Phage Secretion: C-terminals of P1 and P11 interact with N-terminal of P4 to form a complex through which mature phage is secreted.
PII *Replication: Initiates genome replication by nicking the double stranded phage DNA Without P2, could the bacteria still make the phage gene products without replicating the phage DNA? If we remove P2 (and P5), could we use this virus to insert other genes into the bacteria? NOTE: Altering the second half of P2 will affect P10.
PIII *Rounded Tip of Filament: First protein to interact with the E. coli host pilus during infection and last point of contact as phages bud from bacteria. without P3, phage cannot fully escape host. We might change P3 such that the phage was only capable of infecting a different host other than E. coli, if the need arose.
PIV

M13 Design Constraints

The Family Tree: M13's closest evolutionary relatives

Personal tools