User:Emily Suter: Difference between revisions
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I did high resolution microscopy this summer at The Jackson Laboratory with their Institute of Molecular Biophysics. I prepared samples and extracted DNA as well as helped design a computer program to analyze our images, but did not do much PCR or electrophoresis. | I did high resolution microscopy this summer at The Jackson Laboratory with their Institute of Molecular Biophysics. I prepared samples and extracted DNA as well as helped design a computer program to analyze our images, but did not do much PCR or electrophoresis. | ||
=== | ===Module 3 Notes and Report Summary=== | ||
# Original Proposal: "We want to test the effect of chondrocyte cell concentration on the proliferation of chondrocytes in dense alginate beads. Using 3% alginate, we plan on comparing high and low cell concentrations to hopefully extrapolate information about nutrient transport and cellular competition/cellular viability in dense scaffolds." | |||
# ELISA protein test: The ELISA will allow us to compare the concentrations of Collagen Type I and Type II. This will help us determine the number of differentiated cells vs. the number of dedifferentiated cells. From the ratio of Type I to II, we can also maybe determine the relative number of cells in each sample and thereby compare how well the high and low concentrations grew compared to each other. | |||
# | |||
Revision as of 12:13, 30 April 2009
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Registration/Questionnaire: 20.109 Spring 2009
Last Name
Suter
First Name
Emily
Preferred name (if not first)
Course/Minor
20/2
Year of Graduation
2011
Telephone #
916.524.8915
emsuter AT mit DOT edu
Potentially Relevant Background
| Have you taken/are you taking... | Answer yes/no/when |
| 7.05/5.07 (Biochemistry) | yes, Fall 08 |
| 7.06 (Cell Biology) | next semester |
| 7.03 (Genetics) | currently taking |
| 7.02 (General Biology Lab) | no |
| 5.310 (General Chemistry Lab) | no |
| Do you have experience with... | Answer yes/no/type |
| Cell culture (microbial/mammalian/yeast?) | some |
| Molecular biology (electrophoresis, PCR, etc) | minimal |
Please briefly describe any previous laboratory experience
I did high resolution microscopy this summer at The Jackson Laboratory with their Institute of Molecular Biophysics. I prepared samples and extracted DNA as well as helped design a computer program to analyze our images, but did not do much PCR or electrophoresis.
Module 3 Notes and Report Summary
- Original Proposal: "We want to test the effect of chondrocyte cell concentration on the proliferation of chondrocytes in dense alginate beads. Using 3% alginate, we plan on comparing high and low cell concentrations to hopefully extrapolate information about nutrient transport and cellular competition/cellular viability in dense scaffolds."
- ELISA protein test: The ELISA will allow us to compare the concentrations of Collagen Type I and Type II. This will help us determine the number of differentiated cells vs. the number of dedifferentiated cells. From the ratio of Type I to II, we can also maybe determine the relative number of cells in each sample and thereby compare how well the high and low concentrations grew compared to each other.
