User:Emmalee Jones/Notebook/Lab Notebook/2010/04/07: Difference between revisions
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== | ==Prep and Plan== | ||
* | * Made two flow cells, one with a rinsed slide one not rinsed | ||
<br> | |||
1) Background images<br> | |||
a. PLL without anything | |||
<br>b. PLL with liposomes | |||
<br> | |||
2) PLL surface - Determine a recipe that will work every time knowing these facts: | |||
<br>a. I have to coat slides and slips with PLL before assembling flow cell | |||
<br>b. I could make up a stock of flow cells OR a stock of PLL coated slides and slips OR just cleaned slides and slips | |||
<br>c. I think what is taking quite a bit of time is DRYING the slides and slips after coating so it seems that what takes the most amount of time is the lysine step - I think I need to make up a stock that is PLL coated at least, and maybe assemble on the day of? (Assembling flow cell part doesn't really take that long itself) | |||
<br>i. Put tape on each side, smoosh (technical term) with the edge of the retracted razor blade | |||
<br>ii. Cut the pieces of tape down with razor blade (hard to make a clean break at the edge to grab and pull off) | |||
<br>iii. Put slip on, and smoosh again, making very sure to smoosh the edges well | |||
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Revision as of 06:57, 15 April 2010
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Prep and Plan
1) Background images
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