User:Emmalee Jones/Notebook/Lab Notebook/2010/04/30: Difference between revisions
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== | ==Finding Even Coverage Concentration== | ||
* | * Made 0.1 mM total lipid dilution in PEM | ||
* Used this to make lipid flow cell solution | |||
* Still not covering the entire slide, so made up a new solution that is 0.5 mM, put in a flow cell | |||
* This one has even coverage! So that is when I make a 0.5 mM dilution in PEM, then put that into a half and half lipid motility solution for the flow cell. I only made a half batch of it, and that was plenty to run a few flow cells. | |||
<br>Half batch: | |||
25 μL liposomes | |||
1.25 μL AF | |||
0.5 μL Glucose | |||
23.3 μL PEM | |||
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Revision as of 14:35, 30 April 2010
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Finding Even Coverage Concentration
25 μL liposomes
1.25 μL AF
0.5 μL Glucose
23.3 μL PEM
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