User:Emmnanuel Quiroz: Difference between revisions
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# http://arjournals.annualreviews.org/doi/full/10.1146/annurev.genet.38.072902.091500?cookieSet=1 (recombination proteins in yeast - helpful detailed descriptions of Rad50 and many other proteins) | # http://arjournals.annualreviews.org/doi/full/10.1146/annurev.genet.38.072902.091500?cookieSet=1 (recombination proteins in yeast - helpful detailed descriptions of Rad50 and many other proteins) | ||
# http://www.nature.com/ng/journal/v29/n4/full/ng778.html (genes required for ionizing radiation resistance in yeast) | # http://www.nature.com/ng/journal/v29/n4/full/ng778.html (genes required for ionizing radiation resistance in yeast) | ||
# http://genomebiology.com/2006/7/9/233(Functional genomics of the yeast DNA-damage response) | # http://genomebiology.com/2006/7/9/233 (Functional genomics of the yeast DNA-damage response) | ||
===Potential Gene Targets=== | ===Potential Gene Targets=== | ||
Revision as of 13:46, 29 April 2009
I am a new member of OpenWetWare!
Contact Info
- Emmanuel Quiroz
- Biological Engineering
- 416 Beacon St
- Address 2
- Boston, MA, 02115
- equiroz AT mit DOT edu
I work in the Wittrup Lab at MIT. I learned about OpenWetWare from Class, and I've joined because To access my classes material.
Chernobyl Fungi Proposal
Topic
Recent studies have discovered fungal species that survive and proliferate in environments of ionizing radiation such as the Chernobyl reactor. These fungi have very efficient DNA repair gene systems that are homologous to Humans. These fungal systems need to be further studied and analyzed through microarrays to provide the possibly of radiation-based cancer therapies.
Research Goals
- Map the DNA repair system of these efficient fungal species
- Compare the fungi system to that of Humans for possible radiation-based cancer therapies.
References
- Ionizing radiation: how fungi cope, adapt, and exploit with the help of melanin
Fungi are seen to proliferate in environments of ionizing radiation such as the Chernobyl reactor and on the outer shell of space craft. These fungi show an increase in melanin expression which suggest that melanin can function in energy capture and prevent DNA damage. It was also seen through microarray analysis that exposure to radiation caused an up regulation in DNA repair genes. Interestingly, many of the radiation resistance genes share significant homology with human genes that might be exploited in the development of novel radiation-based cancer therapies. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VS2-4TS239S-2&_user=501045&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=facff3af8627e0e8bf35887a67b6bcab
- Fungi and Ionizing Radiation from Radionuclides
Radionuclides, atoms with unstable nuclei due to excess energy that undergo radioactive decay, have provided interesting clues as to gene regulation since the Chernobyl power plant accident. Researchers have focused on various forms of fungi as a model of these effects because its large surface area allow for greater uptake capacity of nutrients and make it preferential to surround host plants. Through broad microarray analysis of various fungal stains, short term exposure of yeast to gamma and X radiation has been shown to elicit the up-regulation of genes involved in DNA cell repair, cell rescue defense, and cell fate and metabolism. Cladosporium cladosporioides and Penicillium roseopurpureum (saprotrophic micro-fungi) in particular were shown to over grow on carbon-based radioactive debris, and while radionuclides were incorporated into the cytoplasm, they were highly absorbed in ion-exchange sites (i.e. the cell membrane). Additionally, intense radiation of soil fungal communities led to a dominance of melanized fungal species, indicating a potential radiological protective mechanism in the pigment.
Dighton, John; FEMS Microbiol Lett 2008 Vol. 281 Iss. 2 p. 109 - 20ppublish http://www3.interscience.wiley.com/cgi-bin/fulltext/119404367/HTMLSTART
Other Research
- Einstein Researchers' Discovery of "Radiation-Eating" Fungi Could Trigger Recalculation of Earth's Energy Balance and Help Feed Astronauts
- http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VS2-4TS239S-2&_user=501045&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=facff3af8627e0e8bf35887a67b6bcab
- Chernobyl effect: growth characteristics of soil fungi Cladosporium cladosporioides (Fresen) de Vries with and without positive radiotropism -- Zhdanova, N Fomina, M Redchitz, T Olsson, S Polish Journal of Ecology [Pol. J. Ecol.]. Vol. 49 2001 Vol. 49 Iss. 4 p. 309 - 318
- Fungi from Chernobyl: mycobiota of the inner regions of the containment structures of the damaged nuclear reactor Zhdanova, NN Zakharchenko, VA Vember, VV Nakonechnaya, LT Mycological Research [Mycol. Res.]. Vol. 104 2000 Vol. 104 Iss. 12 p. 1421 - 1426
- http://www.nature.com/bjc/journal/v90/n12/full/6601878a.html (RAD51 in cancer cells)
- http://www.nature.com/onc/journal/v24/n23/full/1208515a.html (XP variant HR pathway)
- http://vera.mit.edu:8080/multi/ML?a=fullrecord&set=000861&record=17 (rad50 involved in synaptonemal complex formation, recombination, and homolog pairing)
- http://arjournals.annualreviews.org/doi/full/10.1146/annurev.genet.38.072902.091500?cookieSet=1 (recombination proteins in yeast - helpful detailed descriptions of Rad50 and many other proteins)
- http://www.nature.com/ng/journal/v29/n4/full/ng778.html (genes required for ionizing radiation resistance in yeast)
- http://genomebiology.com/2006/7/9/233 (Functional genomics of the yeast DNA-damage response)
Potential Gene Targets
- repair (RAD50, RAD51)
- recombination (HRP1)
- chromosome stability (CHL1, CTF4)
- endocytosis (VID21)
- ubiquitin degradation (GRR1)
- transcription (BUR2)
