User:Emzodls

20.109 Spring 2007

Last Name

de los Santos

First Name

Emmanuel Lorenzo

Nickname

Emzo

Course/Minor

20

Year of Graduation

2009

Email

emzodls AT mit DOT edu

Subjects this Term

20.109
7.05
6.001
6.041
21F.101

Module 1 : M13 Genome Re-engineering

M13 Redesign Ideas

Gene	function	design ideas
I	assembly	modify genes I, IV and XI to allow other useful proteins to attach to the complex (more flexibility in phage modification
II	replication of DNA + strand	add some kind of control to deactivate protein (e.g. sensitivity to heat or a chemical stimuli), this allows better control of the phages
III	phage tail protein	add myc epitope, or another marker that can be used for experimentation
IV	assembly	(see modification for I)
V	binds ssDNA	add tag to track changes, change structure to allow more DNA to fit in the phage
VI	phage tail protein	bind it tighter to phage body, modify protein to allow more modifications to p3
VII	phage head protein	(see p6), make protein 9 more flexible to modifications
VIII	phage coat protein	add myc or another tag (x-ray sensitive, UV sensitive, flourescent)
IX	phage head protein	tag, allow p9 to attach to different kinds of proteins including p3 (makes virus chains)
X	DNA replication	add another way to control phage propagation
XI	assembly	(see modification for I)