User:Eric M. Walters/Notebook/Spring 2012/2012/05/22
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Cyanobacterial acrylate production | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Digestion
SphI-HF was used to digest, in Buffer 4 at 37°C, 45 minutes (10:45-11:30). Heat inactivated at 65°C for 20 minutes.
LigationLigation will be done for 30 minutes at room temperature (12:05-12:35), per Matt's advice. 50 μL, since that's what I've needed before.
Gel after 30 minutes (but continued for another 60):
AmplificationI want to increase the amount of ligation produce I've got to transform with via PCR. 2x50 μL reactions Per 50 μL:
Run overnight to be cleaned, AGEd, and transformed. |