User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/13: Difference between revisions

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===Part 1: PCR===
===Part 1: PCR===
*Protocol as specified in MICB323 Lab Manual pages 8-9
*Protocol as specified in MICB323 Lab Manual pages 8-9
*See link for experimental values used. [http://www.openwetware.org/images/6/6e/090113_Lab_1_Part_1.numbers.pdf]
*See link[http://www.openwetware.org/images/6/6e/090113_Lab_1_Part_1.numbers.pdf] for experimental values used.
*200µL Thin-Walled Tube Numbers:
*200µL Thin-Walled Tube Numbers:
**ES #1: Primer 1 + DNA 1
**ES #1: Primer 1 + DNA 1

Revision as of 15:35, 13 January 2009

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Lab 1: PCR, Modification of PCR Component, and Phage T4 Growth Curve

Part 1: PCR

  • Protocol as specified in MICB323 Lab Manual pages 8-9
  • See link[1] for experimental values used.
  • 200µL Thin-Walled Tube Numbers:
    • ES #1: Primer 1 + DNA 1
    • ES #2: Primer 1 + DNA 2
    • ES #3: Primer 2 + DNA 1
    • ES #4: Primer 2 + DNA 2
  • Tubes put into ice.

Part 2: Modification of PCR Component

  • Protocol as specified in MICB323 Lab Manual page 11
  • See link for experimental values used.
  • Variable chosen to vary: KCl concentration
  • 200µL Thin-Walled Tube Numbers:
    • EM #1: 10X Buffer contains 0.25M KCl
    • EM #2: 10X Buffer contains 0 M KCl
    • EM #3: 10X Buffer contains 0.125M KCl (0.5X the concentration of original)
    • EM #4: 10X Buffer contains 1M KCl (4X the concentration of original)

Part 3: Phage T4 Growth Curve


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