User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/20: Difference between revisions

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===Part 3: Bacteriophage Growth Curve===
===Part 3: Bacteriophage Growth Curve===
Raw data is found here: ([[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx|MS Excel Version]])([[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx.pdf|PDF Table]])([[Media: 090120_Lab_2_Bacteriophage Graph.xlsx.pdf|Graph]]).
Raw data and calculations are found here:  
*[[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx|MS Excel Version]]
*[[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx.pdf|PDF Table]]
*[[Media: 090120_Lab_2_Bacteriophage Graph.xlsx.pdf|Graph]]




Revision as of 16:30, 20 January 2009

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Lab 2: PCR Gel Electrophoresis, PCR: Ligation, Bacteriophage Growth Curve Analysis

Part 1: Electrophoresis of PCR Products

Gel Ingredients:

  • 100mL 1X TAE
  • 1.5g agarose
  • 10µL SYBRsafe

Lanes:

  • 1: SD 1
  • 2: SD 2
  • 3: SD 3
  • 4: SD 4
  • 5: Mass Ruler
  • 6: ES 1
  • 7: ES 2 - 380 bp product appeared on gel
  • 8: ES 3
  • 9: ES 4 - 1000 bp product appeared on gel
  • 10: ES 5
  • 11: Mass Ruler
  • 12: EM 1
  • 13: EM 2
  • 14: EM 3
  • 15: EM 4

Run gel, 120V for 1hr. Link: Gel image.

Part 2: Ligation Reaction

  • Followed protocol in lab manual - pages 25 to 26.

Part 3: Bacteriophage Growth Curve

Raw data and calculations are found here:



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