User:Eric Ma/Notebook/MICB323 Lab Book/2009/01/20: Difference between revisions
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*[[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx.pdf|PDF Table]] | *[[Media: 090120_Lab_2_Bacteriophage_Counts.xlsx.pdf|PDF Table]] | ||
*[[Media: 090120_Lab_2_Bacteriophage Graph.xlsx.pdf|Graph]] | *[[Media: 090120_Lab_2_Bacteriophage Graph.xlsx.pdf|Graph]] | ||
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Calculation of Burst Size: | |||
*Lower step average: 6x10<sup>2</sup> pfu/mL | |||
*Upper step average: 2.5x10<sup>4</sup> pfu/mL | |||
*Average burst size = 2.5x10<sup>4</sup>/6x10<sup>2</sup> = 41.7 | |||
*This is approximately the expected range. (Mrs. Hinze told us average burst size is around 100 for Phage T4 in ''E. coli''. | |||
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Calculation of | |||
Note to self: Calculations - still need to figure out how to display calculations in wiki format. For now, viewer can download excel file to view formulas used for the calculations. | Note to self: Calculations - still need to figure out how to display calculations in wiki format. For now, viewer can download excel file to view formulas used for the calculations. | ||
Revision as of 09:48, 24 January 2009
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Lab 2: PCR Gel Electrophoresis, PCR: Ligation, Bacteriophage Growth Curve AnalysisPart 1: Electrophoresis of PCR ProductsGel Ingredients:
Lanes:
Run gel, 120V for 1hr. Gel Image:
Part 2: Ligation Reaction
Part 3: Bacteriophage Growth CurveRaw data and calculations are found here:
Summary
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